Figure 2 PET-CT images, BNP, HWI and HE staining of myocardial sections of each group on the 7th and 14th day after injection. A: PET-CT images showed high uptake on the 7th and low uptake on the 14th day. (n=6) B: No obvious changes in BNP were seen. (n=8) C: HE staining of myocardial sections showed some myocardial cell destruction in 5-fluorouracil + bevacizumab group on the 14th day after injection (objective × 10). 5-FU + BEV group: 5-fluorouracil + bevacizumab group.
LVPW;d decreased on the 7th and 14th day and IVS;d, EF, FS decreased on the 14th day: As a convenient method to detect the function and structure of the heart, echocardiography was performed before injection and on the 4th, 7th and 14th days after injection to evaluate the changes of left ventricular function and structure in mice. Before and the 4th, 7th and 14th days after injection, there was no obvious cardiac echo change in the echocardiogram of mice in each group. The mitral valve, tricuspid valve and aortic valves were soft and well opened, and there was no obvious reflux signal. There was no obvious change in the heart structure of mice in each group. Compared with the control group, the IVS;d and LVPW;d in the 5-fluorouracil + bevacizumab group became thinner with the progress of the disease, and there was a statistical difference (P <0.05) on the 14th day compared with the control group. The LVID;d and LVID;s slightly increased, but no statistical difference (P >0.05). LVEF and LVFS decreased significantly on the 7th and 14th day compared with the control group. This indicated that 5-fluorouracil and bevacizumab had certain damage to the heart and had a certain correlation with the dosage. As figure 3, part A-G.