Figure 3 LVPW;d decreased on the 7th and 14th day and IVS;d, EF, FS decreased on the 14th day after injection in the 5-fluorouracil + bevacizumab group. (n=10) A: M mode of each group on the 7th day and 14th day. B: IVS;d obviously decreased on the 14th day. C: LVPW;d decreased obviously on the 7th and 14th day. D: LVID;d slightly increased on the 7th and 14th day. E: LVID;s slightly increased on the 7th and 14th day. F: EF obviously decreased on the 14th day. G: FS obviously decreased on the 14th day. H: Spectral doppler and tissue doppler imaging of each group on the 7th day and 14th day. I: No obvious changes in MVE were seen. J: No obvious changes in MVA were seen. K: No obvious changes in MV IVS e’ were seen. L: MV E/A of 5-fluorouracil + bevacizumab group decreased on the 7th and 14th day. M: MV E/e’ slightly increased on the 14th day after injection. 5-FU + BEV group: 5-fluorouracil + bevacizumab group. *: P<0.05. **: P<0.01.
There were no obvious changes in MV E, MV A and MV IVS e’ of 5-fluorouracil+ bevacizumab group were seen, while MV E/e’ slightly increased on the 14th day after injection. MV E/A of 5-fluorouracil + bevacizumab group decreased on the 7th and 14th day, but there was no significant difference (P >0.05). Therefore, we can observe that 5-fluorouracil and bevacizumab had little effect on diastolic function, and they were difficult to detect in conventional echocardiography. As figure 3, part H-M.
Global longitudinal strain decreased at first on the 7th day: In order to verify the application of speckle tracking echocardiography in the diagnosis of chemotherapy induced cardiotoxicity, we used VEVO strain to analyze the left ventricular wall motion of mice in each group. The GLS (global longitudinal strain) of left ventricular in 5-fluorouracil + bevacizumab group was significantly lower than that in the control group (P <0.05) on the 7th and 14th day. The long axis GRS (global radial strain) was lower than that in the control group (P <0.05) on the 14th day. And the GCS (global circumferential strain) was significantly lower than that in the control group (P <0.05) on the 14th day. On the 7th and 14th day, the short axis GRS was lower than that of the control group, but there was no statistical difference (P >0.05). While the long axis GLSR (global longitudinal strain rate) and the long axis GRSR (global radial strain rate) were only slightly lower than that of the control group on the 14th day, but there was no statistical difference (P >0.05). The short axis GCSR (global circumferential strain rate) and the short axis GRSR were lower than that of the control group on the 7th and 14th day, but there was no statistical difference (P > 0.05). As figure 4.