2.1-Experimental animals and groups:
This study used 24 Wistar Albino male rats weighing 240-280 g. Animals were housed in fixed limits at room temperature (24±2°C) with 55%±15 relative humidity and 12 hour/12 hour light-dark cycles. Water and standard rat feed were given ad libitum. Care was taken that all procedures related to animals were completed in accordance with national and international regulations related to animal experiments. The study was completed in Kutahya Health Sciences University Experimental Animal Breeding Research and Application Center, Dumlupinar University Advanced Technology Design, Research and Development Center and Eskisehir Osmangazi University, Central Research Laboratory, Application and Research Center. The study was completed in two stages. The first study received ethics committee permission from Dumlupinar University Animal Experiments Local Ethics Committee. Later, in order to more clearly prove the hypothesis, tissue obtained from the previous study were studied with an electron microscope and for this second ethics committee permission was obtained from Kutahya Health Sciences University Animal Experiments Local Ethics Committee.
The first stage of the study was supported by Dumlupinar University -SRP and the second stage was supported by Kutahya Health Sciences University -SRP.