2.1-Experimental animals and groups:
This study used 24 Wistar Albino male rats weighing 240-280 g. Animals
were housed in fixed limits at room temperature (24±2°C) with 55%±15
relative humidity and 12 hour/12 hour light-dark cycles. Water and
standard rat feed were given ad libitum. Care was taken that all
procedures related to animals were completed in accordance with national
and international regulations related to animal experiments. The study
was completed in Kutahya Health Sciences University Experimental Animal
Breeding Research and Application Center, Dumlupinar University Advanced
Technology Design, Research and Development Center and Eskisehir
Osmangazi University, Central Research Laboratory, Application and
Research Center. The study was completed in two stages. The first study
received ethics committee permission from Dumlupinar University Animal
Experiments Local Ethics Committee. Later, in order to more clearly
prove the hypothesis, tissue obtained from the previous study were
studied with an electron microscope and for this second ethics committee
permission was obtained from Kutahya Health Sciences University Animal
Experiments Local Ethics Committee.
The first stage of the study was supported by Dumlupinar University -SRP
and the second stage was supported by Kutahya Health Sciences University
-SRP.