Patients
Fresh-frozen HBs (n=21) and matched non-tumoral liver tissues (n=9) were
recovered from biobanks. All patients received pre-surgery chemotherapy
according to either SIOPEL (http://www.siopel.org /) or COG
(https://www.childrensoncologygroup.org /) protocols, and they
were followed by clinical examination, imaging tests, and
alpha-fetoprotein dosage. This study was approved by the Ethics
Committees of the A.C. Camargo Cancer Center and GRAACC. Biological
samples were collected after signed informed consent obtained from
parents.