In silico analysis
To explore the pathways in which the genes are involved with, we used
Reactome Pathway Database (https://reactome.org/ - Version 72 Released).
A binomial test is used to calculate the probability for each result and
the p-values are corrected for the multiple testing (Benjamini–Hochberg
procedure) that arises from evaluating the submitted list of identifiers
against every pathway 24,25. We considered
significant, those p-values <0.05. Data were crossed with
other resources such as NCBI, Ensembl, UniProt, KEGG (Gene and
Compound), ChEBI, PubMed and GO.
Pairwise gene expression correlation was calculated using Pearson’s
method and the data was displayed using the heatmap.2 function
(packages: STRINGR, GGPLOT2, GRID, gridExtra, and GGBIPLOT) with the
same setting as the UPGMA previously described.