RNA isolation
RNA samples were obtained from the biobank of the A.C. Camargo Cancer
Center and GRAACC; samples were extracted from tissues using the
QIASymphony DNA Mini kit (QIAGEN ) and the RNeasy Mini Kit
(QIAGEN ), according to manufacturer’s recommendations. The
quality of RNA samples was verified by microfluidics-based
electrophoresis (Bioanalyzer, Agilent Technologies; CA, USA), and only
RNA samples with RNA Integrity Number (RIN) >7.0 were used.