2.2| PATHOLOGICAL AND CYTOLOGICAL EVALUATION
From all necropsied animals (n = 11), multiple organ samples were
collected and fixed in 10% buffered formalin. The material was sent to
the Laboratory of Pathological Anatomy of the Biological Institute in
São Paulo State. The tissue samples were routinely processed, embedded
in paraffin, and sections were stained with hematoxylin and eosin (HE)
and Gram (Kiernan, 2008; Yen et al., 2019).
Since the main pathological findings were located in the abomasum,
direct impressions of the abomasal mucosa were made using glass slides
(Kamatchi et al., 2015). Slides were made in triplicate for each animal
(n = 11), air-dried, and stained by three different techniques, GIEMSA,
Quick Panotic™, and Gram (Merck KGaA, 2014) for cytological evaluation
under optical microscopy.