3.3| BACTERIOLOGICAL AND MOLECULAR FINDINGS
From the anaerobic culture and molecular identification, 71.4% (5/7) of
kids with abomasal bloat signs were positive for C. perfringenstype A. Also, 25.0% (1/4) of kids without abomasal bloat signs were
positive for C. perfringens type A as seen in Table 2. Regarding
the molecular analysis, a total of 71.4% (5/7) of kids with abomasal
bloat signs were positive for both sets of primers (PDC and the 16S rRNA
genes). Likewise, 0.0% (0/4) and 75.0% (3/4) of kids without abomasal
bloat signs were positive for PDC and 16S rRNA genes, respectively. From
the environment samples (n = 7), all were negative for the PDC primer
(specific to C. ventriculi ), and 100.0% (7/7) were positive for
the 16S rRNA primer used to detect Clostridium sp. (Table 2).