Analysis of stress signaling and seed dormancy-related
phytohormones
For each treatment (n=3 plants), 50mg of ground seeds were used to
quantify 2-cis , 4-trans -abscisic acid (ABA), gibberellic
acid (GA3), indole-3-acetic acid (IAA), jasmonic acid (JA), and
salicylic acid (SA). Previously freeze-dried samples were mixed with 500
µL of extraction solvent [2-propanol/H2O/ concentrated
HCl (2:1:0.002, v/v/v)] and then analyzed by HPLC-MS as described in
Pan, Welti & Wang (2010). Because the dynamics of ABA and GA3 controls
the balance between germination and dormancy, the ABA:GA3 ratio was used
as a proxy for seed dormancy: germination balance (Debeaujon &
Koornneef 2000; Finkelstein 2013).