Seed fatty acid concentration
Oil and fatty acid profile contents (C16:0, C18:0, C18:1, C18:2, C18:3, C20:0, C20:1 and C22:1) were determined from approximately 200 mg of seeds from each plant (n=3) suspended in 1 mL of methanol/toluene/H2SO4 solution (100:20:2.5; v/v) containing C17:0 as internal standard (5 µg mL−1). After 1 hour of heating at 80 °C for transmethylation, fatty acid content was determined as described in Marchand et al. (2016).