Botrytis cinerea infection
The fungus was cultivated in potato dextrose agar plates, supplemented
with freeze-dried tomato leaves. Three weeks later, B. cinereaspores were collected from plates in 0.5X potato dextrose broth as
previously described (San Martin et al., 2020). Leaf inoculation was
performed in detached leaves using two 6 µL drops containing a conidia
concentration of 5 x 106 spores/mL per leaflet. Leaves
were maintained in hermetically sealed boxes with 100% of humidity at
21ºC in darkness. Necrotic lesions were evaluated after 5 days.