Abstract: Aim: Coronavirus disease-2019 is an emerging disease that is threatening the world with a rapid increase in cases and deaths since it was first identified in December 2019. Our study aimed to assess the knowledge, practice, and attitude toward COVID-19 among physicians in Jordan and Palestine. Method: This is a cross-sectional study using an online survey conducted in April, 2020. A 36-items survey was developed and invitations were sent to physicians in different health sectors using social media. Results: A total of 454 physicians participated in this study with a mean age 36.2 10.8 years. The mean score of basic knowledge was 4.4 0.8, and there were significant statistical differences between basic knowledge mean scores among physicians in different professional degrees and physicians in different health sectors (P=0.0315, P=0.0137 respectively). Participants showed appropriate practical measures toward COVID-19, where the mean scores of indications for PCR test, indications for home quarantine, self-protection measures, and measures if physician self-suspected of COVID-19 were 5.4 1.4, 5.0 1.0, 6.1 1.1, 9.9 1.1 respectively. The mean score of attitudes toward COVID-19 were 41.5 3.3 and significantly related to the age and different experience years (P=0.0022, P=0.0077 respectively). Conclusion: As the global threat of COVID-19 continues to emerge, it is critical to improve the knowledge, practice, and attitudes of physicians worldwide.