Fig.
1. Enrichment of CD44+CD24− cells within EAC cells using different
concentrations of different chemotherapeutics . A. Dot plot of
flow cytometry using FITC anti-CD44 and PE anti-CD24. Ehrlich cells were
cultured in T-25 tissue culture flasks at a seeding density equals to 2
× 106 cells/flask. When the cells reached 80%
confluency, various concentrations of CDDP, DOX, and PTX were added for
72 hrs. (n = 3 for each concentration). The media was then discarded and
adherent cells were washed by PBS and detached by trypsin-EDTA for 5-10
min and the latter was then inactivated by FBS. Cells were then
collected in tubes and analyzed by flow cytometry for CSCs markers.B. Bar chart of the percentages of
CD44+CD24- cells of the various
chemotherapeutics concentrations used showing the significance levels
against control. CDDP at concentration = 50 µg/mL showed the highest
enrichment of CSCs. One-way ANOVA was done. Data are presented as mean ±
SD. ** p < 0.01, *** p < 0.001. EAC:
Ehrlich ascites carcinoma, FITC: fluorescein isothiocyanate, CD: cluster
of differentiation, PE: phycoerythrin, PBS: phosphate-buffered saline,
FBS: fetal bovine serum, CSCs: cancer stem cells, CDDP: cisplatin, DOX:
doxorubicin, PTX: paclitaxel.