Figure 5. CCK interneurons and pyramidal cells are further compromised by NAM of α5 subunit-containing GABAARs inAppNL-F/NL-F mice. A-B and C-D)Whole-cell current-clamp recordings illustrating sIPSPs and sEPSPs recorded in CCK-SCA cells (A-B) and pyramidal cells (C-D) in CA1 of 12 month old wild-type and AppNL-F/NL-F mice, recorded at a membrane potential of -60 mV in control conditions and after bath-application of α5-SOP002. Bath-application of the α5-SOP002 resulted in a reduction in sIPSP amplitude and frequency, but also increased membrane excitation in both cell types, thus further increasing the aberrant hyperexcitability in the AD model. E-F and G-H) Bar graphs show the overall pharmacological change after applying α5-SOP002 in CCK-SCA and pyramidal cells recorded from wild-type and AppNL-F/NL-F mice at 10-12 months. * P <0.05, **P < 0.01, ***P< 0.001, ****P < 0.0001 (see Table 1 for details).