2.3 Strain engineering
The ackA and poxB gene were knocked out on the basis of
Bgl4(BL21(DE3)ΔarcA ΔldhA ΔatoB ΔpflB )
(Zhao, Li, & Deng, 2018) and performed
according to CRISPR-Cas9 engineering in E. coli(Jiang et al., 2015). The related sgRNA
was designed and shown in Supplementary
3.