5 Conclusions
The soil labile organic C fractions, except for basal respiration, were
generally increased after bio-fertilizer application, which was helpful
to C fixation in soil. The proportions of macro-aggregate and aggregate
stability assessed by mean weight diameter were also significantly
improved by bio-fertilizer. This research showed that bio-fertilizer
supplement had significant positive effects on soil C sequestration and
soil aggregate stability. It was recorded that the supplement of 50%
bio-fertilizer combined with chemical fertilizer was more effective.
Considering the increases of soil C and aggregate stability, it was
believed that bio-fertilizer made markedly contributions to soil
physical and chemical properties.
Therefore, we concluded that 50% bio-fertilizer supplemented chemical
fertilizer could effectively improve soil C fixation and soil aggregate
stability in a sustainable way. This study, however, was a snapshot
record only after two year’s bio-fertilizer application. Thus, long-term
experiment was required to complete assess the effects of bio-fertilizer
application.
Acknowledgements This research was financially supported by the
National Key Research and Development Program of China (2018YFD0300704),
Foundation of He’nan Educational Committee (20B210025), and the
School-based Project of Zhoukou Normal University (ZKNUC2018012).