Figure 1 .A) Normalized plots for exon exons 6. The arrow of the mutated curve C> G, c. 629, differs from that of non-mutated specimens. Figure B. Differentiating plots for variation of exon 6.
Figure. 2 (A) Results of the sequencing of nucleotide changes in exon 2 where the mutation c.44 T> A results in a change in the amino acid L15Q B) Results of the sequencing of nucleotide changes in exon 4, which mutations c.331 G> T lead to change The amino acid is D111Y. C) Results of the sequencing of nucleotide changes in exon 6 where mutation c.629C> G leads to alteration of amino acid A210G.
Figure 3. The pathogenic potential of the TBX5 gene variants by PolyPhen-2. A) Mutation A210G is predicted to be possibly damaging B) Mutation D111Y is predicted to be probably damaging and C) Mutation L15Q is predicted to be benign.
Figure 4. A) Interatomic interactions of the A210G mutant. B) Interatomic interactions of the D111Y mutant. Wild type and mutant are colored in light green  and black arrow also represented as sticks alongside the surrounding residues, which are involved on any type of interactions. C) Color definition for bond type.