Changes in vibrational entropy and normal mode analysis
In order to determine the effects of the mutations in flexible
conformations and stability of the protein tbx5 (PDB Id: 5BQD), we also
used DynaMut, a consensus predictor of protein stability based on the
vibrational entropy changes predicted by ENCoM (an elastic network
contact model) which is a coarse grain NMA (normal mode analysis) method
that considers the nature of the amino-acids and aids in calculating
vibrational entropy changes upon mutations, and the stability changes
predicted by graph-based signatures that are used in mCSM program
(Rodrigues, Pires, & Ascher, 2018) (Table 3).