Changes in vibrational entropy and normal mode analysis
In order to determine the effects of the mutations in flexible conformations and stability of the protein tbx5 (PDB Id: 5BQD), we also used DynaMut, a consensus predictor of protein stability based on the vibrational entropy changes predicted by ENCoM (an elastic network contact model) which is a coarse grain NMA (normal mode analysis) method that considers the nature of the amino-acids and aids in calculating vibrational entropy changes upon mutations, and the stability changes predicted by graph-based signatures that are used in mCSM program (Rodrigues, Pires, & Ascher, 2018) (Table 3).