Histopathological Findings
Direct smears from the exudate of fluid of the lupoid lesion were
stained with special stains Gram, Erlich-Ziehl-Nielsen (EZN), Periodic
Acid-Schiff (PAS), Giemsa for detection of bacteria, mycobacteria, fungi
and leishmania amastigotes, respectively. A punch biopsy
aspiration fluid from lesions was used, and a part of the exudate of
fluid of the lupoid lesion was used for the identification ofLeishmania species by Real Time PCR. Application of real time PCR
that targeted the ITS1 region of Leishmania species using the
DNAs obtained from the lesion of the patient. In this real time PCR,
melting temperature of L. major reference control and sample is
85°C as described previously2.