Allele and genotype frequencies of two SNPs in the second phase
and combined study
To further verify the correlation between two loci (rs3118470 and
rs2104286) and susceptibility to GD, we genotyped three loci in another
350 GD patients and 700 healthy controls. The result of rs2104286 was
consistent with the first stage results (AA genotype: Pc = 0.006,OR = 1.618), while no association was observed for rs3118470. The
combined data showed that the IL2RA /rs3118470 T allele and the TT
genotype (T allele: Pc = 1.490 × 10-3,OR = 1.278; TT genotype: Pc = 2.212 ×
10-3, OR = 1.453), and theIL2RA /rs2104286 A allele and the AA genotype (A allele: Pc= 0.008, OR = 1.322; AA genotype: Pc = 1.754 ×
10-5, OR = 1.636) were significantly associated
with an increased risk for GD (Table 2). The sequencing results were
consistent with the PCR-RFLP genotyping results.