Figure Legends
Figure 1. Patient 1: Excisional biopsy of the lymph node
involved by polymorphous Post-transplant lymphoproliferative disorder. A
- B. Histomorphologic findings of the lymph node biopsy reveal
effacement of the nodal architecture (A. 40x) by a mixed proliferation
of lymphocytes, immunoblasts, and plasma cells (B. 400x). C - F.
Immunohistochemical studies highlight mixed T-cells (C. CD3), B-cells
(D. PAX5), and polytypic plasma cells (E. CD138, F. Kappa, G Lambda).
EBV EBER in-situ hybridization (H) highlights scattered EBV-positive
cells.
Figure 2 . Patient 1: Bone marrow showing involvement by PTLD.
A. Bone marrow aspirate shows increased number of plasma cells (arrow)
and large B-cells (arrowhead) (A). Immunohistochemical studies highlight
both small and large B-cells (2B, CD20).
Figure 3 . Patient 1: Bone marrow is negative for involvement by
PTLD. Bone marrow biopsy shows mildly hypocellular marrow for age
multilineage hematopoiesis (A. 100X and B. 400X). There is no
morphologic support for involvement by PTLD.
Figure 4. Conventional cytogenetics (G-banded karyotype) and
fluorescence in situ hybridization (FISH) studies were performed on the
lymph node involved with PTLD. A. Chromosome analysis revealed
47,XX,+3[2]/46,XX[18]. B. This finding was confirmed by internal
FISH studies with a probe for the centromere of chromosome 3,
which detected three copies of chromosome 3 in 8.3% (25/300) of the
nuclei examined. FISH studies were performed using commercially
available Vysis Centromere 3 (CEP 3) probe (Abbott Molecular, Des
Plaines, IL), cut off value established for trisomy 3 is 1 percent.