Fig.4 TRPA1 regulates the cytokine expression in DNCB-induced AD mice. (A-F) The skin tissues were collected from DNCB-induced mice on day 21 (the last day of the experiment). Total relative mRNA expression levels of IL-1β (A), TNF-α (B), IL-6 (C), INF-γ (D), IL-4 (E), and IL-13 (F) were measured by quantitative RT-PCR analysis and expressed as a ratio to GAPDH. *p <0.05, **p < 0.01, NS, not significant