3.3 TRPA1 regulates the expression of proinflammatory cytokines
and Th2 cytokines in DNCB-induced AD mouse model
Next, we examined the mRNA expression of inflammatory cytokines in the
dorsal skin of
TRPA1-/- mice and WT mice treated with DNCB. As shown
in Figure 4A-F, the DNCB induced a remarkable increase in the
mRNA expression of IL-1β, TNF-α, IL-6, IL-4, IL-13. Most importantly,
the expression of the proinflammatory cytokine, IL-1β, TNF-α, and IL-6
in the skin lesions significantly decreased in DNCB-treated
TRPA1-/- mice
compared to DNCB-treated WT mice (Figure 4A-4C ). Besides, we
found that Th2 cytokine, IL-4, and IL-13 production were significantly
reduced in DNCB-treated TRPA1-/- mice compared to the
WT mice (Figure 4E-F ); nevertheless, no difference in mRNA
expression of IFN-γ was found between groups (Figure 4D ). To
sum up, the trends of cytokines expression might further explain the
amelioration of DNCB-induced AD-like symptoms and skin pathology in
TRPA1 -/- mice.