3.4 TRPA1 deficiency inhibits DNCB-induced infiltration of
macrophages in AD mice
In chronic AD, the increase of macrophage infiltration is closely
related to the degree of skin damage and immune dysfunction. To verify
the effect of TRPA1 on macrophage infiltration in DNCB-induced AD, the
expression of F4/80 in the back skin was analyzed by immunofluorescence
and Western blot. The results showed that the number of F4/80+ cells and
its protein expression significantly increased after repeated skin
stimulation with DNCB. Compared with the WT group, the number of
F4/80+ cells and its protein expression in TRPA1-/-
group decreased (Figure 5A-5B ).
There are two main pathways for macrophage activation, the classical M1
pathway and the alternative M2 pathway. In this study, we explored the
effect of TRPA1 on the infiltration of M1 and M2 macrophages. The
results showed that
the
expression of
iNOS+ and CD206
protein induced by DNCB were significantly increased and in the TRPA1-/-
group, the expression of iNOS+ and CD206 protein
decreased compared with the WT group (Figure 5A ). These results
suggest that TRPA1 deficiency inhibits the infiltration of M1 and M2
macrophages in the lesions.