3.2 TRPA1 deficiency reduces mast cell infiltration and serum
tIgE in DNCB-induced AD mouse model
Mast cell infiltration and hyperproduction of IgE are the main features
of AD. In this study, we used toluidine blue to stain the mast cells
from dorsal and ear tissue sections. The results showed higher dermal
mast cell numbers in DNCB-treated WT mice compared to DNCB-treated
TRPA1-/- mice (Figure 3A-3D ). Besides, we
assessed the levels of total IgE in the serum from mice collected on the
last day of the experiment. The results showed that the level of serum
total IgE in the serum was significantly increased by repeated DNCB
stimulation in dorsal and ear; yet, no significant difference was
observed between groups (Figure 4E ).