Fig.3 Effects of TRPA1 deficiency on skin mast cell infiltration and serum tIgE in DNCB-induced AD mice. (A-B) Toluidine blue staining of dorsal and ear skin lesions was used to identify mast cells. The black arrows indicated the stained mast cells. Scale bar, 100 μm. (C-D) The number of mast cells per site, results are expressed as the mean ± SEM.(E) Blood was collected on the last day of the experiment (n=6), serum levels of tIgE were measured by ELISA (n=6). *p<0.05, **p<0.01,NS, not significant