2.10 Statistical analysis
Statistical analysis was performed using GraphPad Prism software (ver. 7.0 GraphPad Software, San Diego, CA, USA). Results are expressed as mean ± standard error of the mean(S.E.M). Student’st- test was used for comparisons between two groups. One-way ANOVA analyzed differences among multiple groups. p values < 0.05 were considered to be statistically significant.