2.4 Histological and immunochemistry analysis
The ear and dorsal skin were fixed with 4% paraformaldehyde, embedded
in paraffin and cut into 5μm thick sections. Samples were then treated
with Hematoxylin and Eosin (H&E) to detect epidermal thickening and
inflammatory infiltration. Toluidine blue (TB) was used to evaluate the
infiltration of mast cells. The number of mast cells
in five randomly targeted sites was
counted using a light microscope (Nikon Eclipse Ni, Tokyo, Japan) with
×200 magnification.