3.3 TRPA1 regulates the expression of proinflammatory cytokines and Th2 cytokines in DNCB-induced AD mouse model
Next, we examined the mRNA expression of inflammatory cytokines in the dorsal skin of TRPA1-/- mice and WT mice treated with DNCB. As shown in Figure 4A-F, the DNCB induced a remarkable increase in the mRNA expression of IL-1β, TNF-α, IL-6, IL-4, IL-13. Most importantly, the expression of the proinflammatory cytokine, IL-1β, TNF-α, and IL-6 in the skin lesions significantly decreased in DNCB-treated TRPA1-/- mice compared to DNCB-treated WT mice (Figure 4A-4C ). Besides, we found that Th2 cytokine, IL-4, and IL-13 production were significantly reduced in DNCB-treated TRPA1-/- mice compared to the WT mice (Figure 4E-F ); nevertheless, no difference in mRNA expression of IFN-γ was found between groups (Figure 4D ). To sum up, the trends of cytokines expression might further explain the amelioration of DNCB-induced AD-like symptoms and skin pathology in TRPA1 -/- mice.