3.2 TRPA1 deficiency reduces mast cell infiltration and serum tIgE in DNCB-induced AD mouse model
Mast cell infiltration and hyperproduction of IgE are the main features of AD. In this study, we used toluidine blue to stain the mast cells from dorsal and ear tissue sections. The results showed higher dermal mast cell numbers in DNCB-treated WT mice compared to DNCB-treated TRPA1-/- mice (Figure 3A-3D ). Besides, we assessed the levels of total IgE in the serum from mice collected on the last day of the experiment. The results showed that the level of serum total IgE in the serum was significantly increased by repeated DNCB stimulation in dorsal and ear; yet, no significant difference was observed between groups (Figure 4E ).