3.2.2 Bocaparvovirus
The genus Bocaparvovirus contains 25 recognised species that infect many mammalian species (Jager et al., 2021). Three species of feline bocaparvovirus have been described –Carnivore bocaparvovirus 3(feline bocavirus 1), Carnivore bocaparvovirus 4 (feline bocaparvovirus 2) and Carnivore bocaparvovirus 5 (feline bocaparvovirus 3) (Lau et al., 2012; Ng et al., 2014; Zhang et al., 2014). We identified feline bocaparvovirus contigs in 11/23 (47.8%) FPV-case libraries and 19/36 (55.5%) healthy control libraries (Figure 2). Feline bocavirus 1 contigs were detected in 18/36 healthy controls (50%) and in 9/23 (39%) of FPV-cases (39% 9/23) (p=0.4317). Among healthy controls, feline bocavirus 1 was the bocaparvovirus with the most abundant reads. By contrast, feline bocaparvovirus 2 was the most abundant bocaparvovirus detected in FPV-infected cats and was significantly more frequent in these (21.7%, 5/23) than in healthy control cats (2.8%, 1/36; p =0.0291). Finally, 6/23 (26%) FPV-cases and 1/36 (2.8%) healthy control cats had feline bocaparvovirus 3 contigs (p = 0.0114) (Table 1). Our phylogenetic analysis revealed that feline bocavirus sequences from the case and control libraries were scattered throughout the phylogeny. Feline bocaparvovirus 2 and 3 sequences were more tightly clustered, but clades lack sufficient sequence numbers to assess diversity (Figure 3).