3.7 Anelloviridae
Anelloviruses are small single stranded circular DNA viruses in theAnelloviridae family and are the most abundant member of the human eukaryotic virome, comprising around 70% of eukaryotic viruses detected, which include over 700 viruses from 23 families (Gregory et al., 2020; McElvania TeKippe et al., 2012; Ng et al., 2009; Zhang et al., 2016). Until now feline anellovirus reads have been identified in sick animals (Jarošová et al., 2015; Li et al., 2020; Zhang et al., 2014). We observed diverse anellovirus contigs in 65.2% (15/23) FPV-case and 83.3% (32/36) healthy control cats (p=0.1289). Contigs were relatively divergent and shared ~30-90% amino acid sequence similarity. In total >700 anellovirus contigs were detected, of which more than 400 exhibited <50% amino acid similarity to the nearest NCBI virus protein hit. Therefore, read abundance for Anelloviridae was not calculated and because these sequences are so divergent we did not perform a phylogenetic analysis.