3.2.3 Chaphamaparvovirus and Dependoparvovirus
Chaphamaparvovirus is a recently proposed genus in theParvoviridae family, subfamily Hamaparvovirinae that includes species isolated from pigs, dogs, rodent, duck, owl and bat hosts (Palinski et al., 2016; Palombieri et al., 2020; Roediger et al., 2018; Souza et al., 2017; Vibin et al., 2020; Yang et al., 2016). Feline chaphamaparvovirus was recently discovered in the faeces of shelter-housed cats with diarrhoea (Li et al., 2020). We did not detect feline chaphamaparvovirus contigs in any of the FPV-case libraries. However, read abundance data showed the presence of feline chaphamaparvovirus DNA in FPV-case samples at relatively low levels (maximum 25 reads in the metagenomic library). By contrast, in the healthy controls feline chaphamaparvovirus contigs were detected in 6/36 libraries (p = 0.0724) (Figure 2). In the six sequencing libraries, abundance values ranged from 61 to 6,749 RPM for the metagenomic libraries and 87 and 4,648 RPM for the metatranscriptomic libraries.
Dependoparvovirus, another genus in the Parvoviridaefamily, includes the species Feline dependoparvovirus, previously detected in a single cat in the same shelter cat population in which feline chaphamaparvovirus was discovered (Li et al., 2020). HereFeline dependoparvovirus DNA was detected in 1/23 FPV-case libraries and 5/36 healthy control libraries (p=0.3886).