3.1 Overview
For FPV-cases, a total of 23 metatranscriptomic and 42 metagenomic (22
DNA and 20 cDNA) sequencing libraries were generated, while for the
healthy control cats, we obtained 36 metatranscriptomic and 72
metagenomic (36 DNA and cDNA) sequencing libraries. Results from one
FPV-case sample were excluded due to poor sequencing quality in both
metatranscriptomic and metagenomic libraries. Sequencing produced
2,316,187,631 paired end reads after filtering, including 1,174,728,799
from metatranscriptomic and 1,141,458,832 from metagenomic sequencing.De novo assembly generated 9,492,829 contigs from the
metatranscriptomic sequencing libraries and 538,922 contigs from the
metagenomic sequencing libraries. Viral read abundance calculated by
CCMetagen and grouped by taxonomy classification is depicted in Figure
1. Enteric virus co-infections detected in FPV-cases and healthy control
cats are summarised in Figure 2.