Objective: To analyze covid-19-associated test results and optimize screening procedure for obstetrics and gynecology outpatients Design: Single center retrospective study. Setting: Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei province, P.R. China. Population or Sample: A total of 1,051 obstetrics and gynecology outpatients were included in the study from March 12 to April 26, 2020. Methods: We collected medical records, COVID-19-associated test results and reported the visit procedure for obstetrics and gynecology outpatients. Main outcome(s) and measure(s): We analyzed covid-19-associated test results and followed up the patients. Results: After reviewing the visit procedure, a total of 1051 obstetrics and gynecology outpatients were included in the study, and a series of COVID-19 tests were conducted, which included the SARS-CoV-2 nucleic acid test, chest CT scan and SARS-CoV-2-specific serological test. The SARS-CoV-2 nucleic acid test was negative in all 1051 patients. None of the patients admitted to the hospital experienced SARS-CoV-2 infections during hospitalization. Seventy-six cases were diagnosed with abnormal uterine bleeding-related disease (excluding endometrial malignancy), 2019-nCoV antibody positive rate was 6.579%, which is greater than the average positive rate of 3.974%, and the positive rate among patients with other diseases. No nucleic acid positivity was found in the follow-up of 9 IgM+ (and IgG+) patients. Conclusions: As the peak of the epidemic has passed, a more optimized screening procedure is required to avoid hospital infections.