Larval sampling and trait measurements in juveniles
Acropora spathulata larvae were sampled into 100% ethanol after
rearing at 27.5°C ~2.5 days after fertilization. DNA
from tissue samples were extracted using a modified SDS protocol
previously used for sequencing of single coral eggs (Quigley, Willis, &
Bay, 2017). Juvenile growth (percent change in area), bleaching (percent
change in coloration), and survival (alive or dead) were quantified
after 70 days of experimental exposure to 27.5°C and 31°C, and a
detailed assessment of this physiological data can be found in (Quigley
et al., 2020).