Larval sampling and trait measurements in juveniles
Acropora spathulata larvae were sampled into 100% ethanol after rearing at 27.5°C ~2.5 days after fertilization. DNA from tissue samples were extracted using a modified SDS protocol previously used for sequencing of single coral eggs (Quigley, Willis, & Bay, 2017). Juvenile growth (percent change in area), bleaching (percent change in coloration), and survival (alive or dead) were quantified after 70 days of experimental exposure to 27.5°C and 31°C, and a detailed assessment of this physiological data can be found in (Quigley et al., 2020).