Laboratory Measurements:
Total protein (Total protein Gen2), albumin (Albumin Gen2) and immunoglobulin (Tina-quant Gen2 IgA, Tina-quant Gen2 IgM, Tina-quant Gen2 IgG) test kits were purchased from Roche Diagnostics. Measurements were performed using the Cobas 8000 c702 autoanalyzer (Roche Diagnostics, GmbH). Bromocresol green (BCG) method was used for albumin measurement. Total protein was analyzed by biuret method and Immunoglobulins were analyzed by immunoturbidimetric method. The gamma globulin fraction was directly obtained by protein electrophoresis which was performed by Capillarys 3 Octa automated capillary electrophoresis system (Sebia, Paris, France).