Laboratory Measurements:
Total protein (Total protein Gen2), albumin (Albumin Gen2) and
immunoglobulin (Tina-quant Gen2 IgA, Tina-quant Gen2 IgM, Tina-quant
Gen2 IgG) test kits were purchased from Roche Diagnostics. Measurements
were performed using the Cobas 8000 c702 autoanalyzer (Roche
Diagnostics, GmbH). Bromocresol green (BCG) method was used for albumin
measurement. Total protein was analyzed by biuret method and
Immunoglobulins were analyzed by immunoturbidimetric method. The gamma
globulin fraction was directly obtained by protein electrophoresis which
was performed by Capillarys 3 Octa automated capillary electrophoresis
system (Sebia, Paris, France).