2.8.1 Cell counting
The number of immunopositive DRG neurons was determined by counting
neurons that contained a nucleus and showed strong signal intensity in
the cytoplasm >2.5-fold above the noise level in the same
sections, as described previously
(Nakamura et al., 2008). The total number
of immunopositive neurons was calculated from two to three
segment/level-matched DRG sections from 2-3 mice of each genotype.