3.6 CPV-2 undergoes negative selective pressure on its evolution
The dN/dS ratio was determined from the alignment of the individual
NS1/NS2 and VP1/VP2 genes from the available CPV-2 genomes. Both SLAC
and FEL analyses indicated that overall the CPV-2 gene has undergone
negative selective pressure (dN/dS < 1), but potentially
positive selection sites were also evident in both the NS1/NS2 and
VP1/VP2 genes (Supplementary Table S5).