Protein complex analysis
To predict the most important protein complexes, protein–protein interactions for differentially expressed TFs (DE-TFs) were retrieved from STRING database (Szklarczyk et al., 2019). These interactions were filtered by transcriptomic data to keep only meaningful interactions. Finally these interactions along with expression data were loaded into the cystoscope software (Saito et al., 2012) and a protein–protein interaction network was constructed. This network was analyzed by MCODE software (Bader & Hogue, 2003) in the Cytoscape software environment. Complexes with scores more than two were considered as significant protein complexes.