PCR
PCR was performed with DNA isolated from the blood, grass hay, and maize
silage samples using EconoTaq PLUS (Lucigen, WI, USA) and the OaPV
primers (see Table 1) according to the manufacturer’s instructions.
Conditions for PCR were as follows: 94°C for 2 min; 35 cycles at
94°C for 30 s, 58°C for 30 s, and
72°C for 20s; final extension at 72°C for 5 min. All the amplicons were
sequenced.