Genome Sequencing and Phylogenetic Analysis
The PCR products were purified with a Wizard DNA Clean-Up System (Promega), then sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit (AB) and analyzed with an ABI 3730XL DNA Analyzer. The BioEdit program (version 7.1.3.0) and ClustalW alignment algorithm (version 1.83) were initially used to compare and align nucleotide sequences. Phylogenetic trees were inferred using the neighbor-joining method with MEGA software (version 7.0.26), and bootstrap values were tested with 1000 replications[16]. The evolutionary distances were computed using the Maximum Composite Likelihood method and the units represent the number of base substitutions per site.