Sample Collection
Between 2013 and 2018,
AIV
surveillance according to the China CDC guidelines was conducted in
Anhui province. Environmental samples from LPMs were collected, which
included poultry drinking water, feces, wipes from the surfaces of cages
and boards used for the slaughtering of birds. These samples were stored
in a viral transport medium composed of 15 μg/mL
amphotericin B, 100 units/mL
penicillin G, 50 μl/mL
streptomycin
and 1% bovine serum albumin in Hank’s balanced salt solution (pH 7.4).
Under refrigeration, these sample were transferred to the Laboratory of
Influenza Surveillance immediately, where they were stored prior to
further examination.