Molecular Analyses
The representative sequences used in this study for phylogenetic comparisons and molecular analyses were obtained from the Global Initiative on Sharing Avian Influenza Data (www.gisaid.org) and the GenBank database (www.ncbi.nlm.nih.gov). The Influenza Virus Sequence Annotation Tool (https://www.ncbi.nlm.nih.gov/genomes/FLU/annotation/) was used to predict protein sequences encoded by the input sequences. The potential N-glycosylation sites were predicted using the NetNGlyc 1.0 web server (http://www.cbs.dtu.dk/services/NetNGlyc/). By default, predictions were carried out only on the Asn-Xaa-Ser/Thr sequons.