Protein localization analysis
The transgenic plants of KCS5-GFP, KCS7-GFP, KCS10-GFP, KCS15-GFP , KCS20-GFP and KCS21-GFP were constructed by fusing GFP with genomic sequences of KCS5, KCS7, KCS10, KCS15 , KCS20 and KCS21 driven by their native promoters. The flower buds of the transgenic plants were harvested and the anthers were isolated. The isolated anthers were scanned without fixation with Zeiss LSM510 confocal scanning microscope.