Supplement Data
Figure S1. Reverse transcription polymerase chain reaction (RT-PCR) expression analysis of eleven KCS genes in root, stem, leaf and flowers.
Figure S2. RT-PCR analysis of kcs7 , kcs15 ,kcs21 single mutants and kcs7/15/21 triple mutant.
Figure S3. Phenotypes of single, double and triple mutants ofkcs7 , kcs15 and kcs21 . a and b, Plants, anthers and pollen grains of kcs7 , kcs15 , kcs21 single mutants and kcs7 /15 /21 triple mutant. Black arrows indicated aborted pollen grains in kcs15 andkcs7 /15 /21 triple mutants. Bars= 20 μm. c, Cell biological analyses of WT and kcs7 /15 /21 triple mutant anthers using semi-thin transverse sections. Bars= 20 μm. d, Pollen grains from the single, double and triple mutants of theKCS7 , KCS15 and KCS21 genes visualized under scanning electron microscope. Scale bar for huge pollen 50 μm; for single pollen 10 μm.
Figure S4. Width of kcs7 /15 /21 triple mutant and wild-type pollen when placed in a PEG 3350 series. Each point represents the average width of 100 pollen grains.
Figure S5. kcs7 /15 /21 triple mutant and wild-type in vivo pollen tube growth. a, Emergence of pollen tube with aniline blue staining. Scale bar represents 10 um in figures of 10, 20, 30 and 45 min, 80 um in figure of 1 hour, 100 um in figure of 2 hour. b, The length of pollen tubes at different hours.
Supplemental Table 1. Primers used in this study