2.10 Animals
Male C57BL/6 mice were purchased from the Model Animal Research Center of Nanjing University (Nanjing, China) and were allowed to acclimate to the facilities for at least 7 days. All mice were housed under standard laboratory conditions of temperature at 21 ± 2 °C, relative humidity 50 ± 15%, and 12 h light/darkness cycles, with water and food freely available. Animal studies are reported in compliance with the ARRIVE guidelines (Kilkenny et al. , 2010) and the editorial on reporting animal studies (McGrath et al. , 2015) with the recommendations made by the British Journal of Pharmacology . All experimental procedures and methods in this research were approved by the Institutional Animal Care and Use Committee of Wenzhou Medical University.