Figure 8. Promotion of angiogenesis and proliferation on wound skin tissues. (A ) Representative immunofluorescence images of CD31 (green) and DAPI (blue) staining of wound skin tissues. White arrows indicate the newly formed blood vessels. Scale bars = 300 µm. (B ) Quantitative analysis of the number of blood vessels per mm2. (C ) Immunohistochemistry (IHC) staining of PCNA positive cells in wound skin tissues. (D ) The numbers of PCNA positive cells. *P < 0.05,**P < 0.01,***P < 0.001;##P < 0.01,###P < 0.001 vs. FGF2 group; n.s., no significance. All values displayed are means ± SEM of 5 independent experiments. (E ) Schematic illustration of the designed PEG-FGF2 conjugates that can promote cell proliferation, migration and angiogenesis via FGFR-ERK signing pathway in cutaneous wound.