2.10 Animals
Male C57BL/6 mice were purchased from the Model Animal Research Center
of Nanjing University (Nanjing, China) and were allowed to acclimate to
the facilities for at least 7 days. All mice were housed under standard
laboratory conditions of temperature at 21 ± 2 °C, relative humidity 50
± 15%, and 12 h light/darkness cycles, with water and food freely
available. Animal studies are reported in compliance with the ARRIVE
guidelines (Kilkenny et al. , 2010) and the editorial on reporting
animal studies (McGrath et al. , 2015) with the recommendations
made by the British Journal of Pharmacology . All experimental
procedures and methods in this research were approved by the
Institutional Animal Care and Use Committee of Wenzhou Medical
University.