Figure 6. Effect of PEG-FGF2 conjugates to promote angiogenesisin vitro and ex vivo. (A ) Capillary-like tube formation was assessed by Matrigel angiogenesis assay in HUVECs. Cells were cultured in HG (33 mM) in the presence of FGF2 or PEG-FGF2 conjugates (10 ng·mL-1) for 72 h. (B ) Representative images of aortic rings from C57BL/6 mice. Dotted lines indicate the visual field of sprouts. Quantitative analysis of tube length in (C ) and the number of sprouts in (D ). Scale bars = 300 μm. *P < 0.05,**P < 0.01,***P < 0.001 vs. control group;#P < 0.05 vs. FGF2 group. All data are displayed as means ± SEM of 5 independent experiments.