2.7 Protein stability in wound fluid
Wound fluid was collected as previously described (Grinnell et al. , 1992). The stabilities of FGF2 and PEG-FGF2 conjugates were investigated in wound fluids as previously described (Olekson et al. , 2015). Briefly, FGF2 and PEG-FGF2 conjugates were added to wound fluids collected within 1 day after scalding and further incubated for 48 h at 37 °C. After centrifugation at 12000 rpm, supernatants were collected for bioactivity test. The residual amounts of FGF2 retained for FGF2 and the three PEG-FGF2 conjugates were quantitatively analyzed by ELISA.