Ionomic analysis
Hydroponically grown 1-week-old plants were treated with NS containing 0
or 50 mM NaCl for 1 week in a growth chamber under a 16-h photoperiod.
Samples were harvested and oven-dried at 80 °C for 16 h. Dried tissue
samples were used to determine ion elements of K, Mg, Na, Ca, and P by
inductively coupled plasma‐optical emission spectroscopy (ICP-OES,
Optima 700 dv, Perkin Elmer, USA) at the Central Laboratory, Kangwon
National University. Xylem sap was collected from 1-week-old rice
seedlings grown in half-strength NS. One hour after starting the 0 or
50mM NaCl treatments, xylem sap exuded from the cut surface of leaf
sheath was collected by micropipette into a 1.5 ml E-tube. The amount of
collected sap was weighted and the Mg, Na, and K ions were determined.