Hydrogen peroxide (H2O2)
analysis
To observe H2O2 production in leaf
tissues of the sitl1 and WT, histochemical DAB staining was
conducted as described (Hacham et al., 2011). After salinity stress
treatment for 1 weeks, detached leaves were incubated in DAB
(3,3′-diaminobenzidine) staining solution at 25 °C for 5 h. Bleaching
solution was used to remove chlorophylls of the leaf samples and images
were captured by light microscopy (DM500, Leica, Germany).
H2O2 content in root and leaf tissues
was quantitated as described in Gay and Gebicki (2012). The absorbance
was measured at 560 nm using a SPECTROstarNano. A standard curve of
H2O2 using linear regression analysis
was used to calculate the sample H2O2concentration.