Standard curve
Tg reference calibrators of 10 ng/mL, 50 ng/mL, 100 ng/mL, 200 ng/mL, and 300 ng/mL were added to Tg-POCT test paper as a drop of 60 µL. After reaction at 30℃ for 5 min, the test was performed. The T/C value, which was the signal value, was measured five times and CV was counted. According to the statistical method, T/C ratio of the detected sample was taken as the ordinate, and the concentration of Tg standard was taken as the X-coordinate. The equation was established, and the standard curve was synthesized. Linear regression coefficient was 0.976. The high value was partly due to the hook effect. Overall, the curve was linear and smooth.( Table1. standard curve Figure10. standard curve)
Table1. standard curve