Stability and degree of precision
The 60 µL Tg reference standards (10 ng/mL, 300 ng/mL) were added to the test strip, and the fluorescence values of the quality control line and detection line were read on the immunoassay machine after reaction at 30℃ for 5 min, and the measurements were repeated 10 times. The reagent was placed at 4℃ for 6 months and tested again. The test was repeated 10 times. The CVs in 10 ng/mL point test batch were 9.62%, 13.05%, 7.14%, and 9.43% in 300 ng/mL. Considering that the fluctuation of fluorescence values in the low concentrations were higher than those in the high concentrations owing to human error and other reasons, it was normal that the low concentration value CV was higher than the high concentration CV value. In conclusion, the Tg-POCT reagent has good stability and degree of precision.( Table2. stability and degree of precision)
Table2. stability and degree of precision