Specificity
Take the prepared standard product thrombopoietin (TPO) and the corresponding standard product Tg and test paper at the same time. The cross-reaction rate (CR%) at each concentration was calculate as TPO/Tg × 100%. Within the concentration range used in this study, the cross-reaction rate with TPO was less than 0.1%, indicating that there was no cross-reaction.
Minimum detection limit
The 60 µL standard blank points were added onto the test strip. After reaction at 30℃ for 5 min, the test was repeated 10 times. The T/C ratio was calculated and the concentration was measured using the standard curve. The mean value of T/C was 0.062, which was converted to 0.02 ng/mL on the standard curve. This value was the sensitivity of the test group.( Table3. Minimum detection limit)
Table3. Minimum detection limit