Stability and degree of
precision
The 60 µL Tg reference standards (10 ng/mL, 300 ng/mL) were added to the
test strip, and the fluorescence values of the quality control line and
detection line were read on the immunoassay machine after reaction at
30℃ for 5 min, and the measurements were repeated 10 times. The reagent
was placed at 4℃ for 6 months and tested again. The test was repeated 10
times. The CVs in 10 ng/mL point test batch were 9.62%, 13.05%,
7.14%, and 9.43% in 300 ng/mL. Considering that the fluctuation of
fluorescence values in the low concentrations were higher than those in
the high concentrations owing to human error and other reasons, it was
normal that the low concentration value CV was higher than the high
concentration CV value. In conclusion, the Tg-POCT reagent has good
stability and degree of precision.( Table2. stability and degree of
precision)
Table2. stability and degree of
precision