Media

The semi-defined medium for the pre-culture contained 9.00 g/L glucose, 3.00 g/L KH2PO4, 4.58 g/L K2HPO4, 0.30 g/L peptone, 0.15 g/L yeast extract, 0.75 g/L sodium citrate dihydrate, 0.30 g/L MgSO4 7H2O, 0.03 g/L CaCl2 2H2O, 1.35 g/L (NH4)2SO4, 1.11 g/L NH4Cl, 50 mg/L kanamycin and 150 µL/L of a solution containing 40.00 g/L FeSO4 7H2O, 10.00 g/L MnSO4 H2O, 10.00 g/L AlCl3 6H2O, 7.30 g/L CoCl2 6H2O, 2.00 g/L ZnSO4 7H2O, 2.00 g/L NaMoO4 2H2O, 1.00 g/L CuCl2 2H2O, 0.50 g/L H3BO3. For bioreactor cultivations, defined minimal media according to DeLisa, Li, Rao, Weigand, and Bentley (1999) was used, with glucose as carbon source. The initial glucose concentration was 20 g/L and the substrate feed had a glucose concentration of 400 g/L.