Analysis of lysis
Under the assumption that released DNA is proportional to the amount of
lysed cells, quantification of lysis was adapted from Klein et al.
(2015). For our calculations, we assumed a cellular DNA content of 31
mg/g, which was taken from literature (Neidhardt & Umbarger, 1996).
Double strand DNA in the culture supernatant was measured in triplicate
with the Quant-iT™ PicoGreen® dsDNA Assay Kit (Thermo
Fisher). The accumulation of DNA was corrected with a degradation rate
as described in Supporting Information 3. The amount of lysed cells
(xl ) was calculated according to Klein et al.
(2015) and expressed as percent of total biomass concentration as shown
in Equation 3: