Identifying responsible chromosomes of ‘Blue’, ‘Yellow’, ‘Spot’ and ‘Red cocoon’ phenotypes in BC1 individuals
BC1 individuals, which has any one of four morphological traits, ‘Blue,’ ‘Yellow,’ ‘Spot’ and ‘Red cocoon,’ were picked up in order to identify responsible chromosomes for each trait. Genetic markers designed for scaffold linkage analysis were utilized (see Table S4). DNeasy Blood and Tissue kit (QIAGEN) and MightyAmp™ DNA Polymerase Ver.3 (TaKaRa) was used for DNA extraction and genomic PCR of BC1 individuals, respectively. The genomic PCR program was as follows: 2 min at 98°C and 40 cycles of 10 s at 98°C, 15 s at 60°C and 1 min at 68°C.