Correlation based clustering between hormones and metabolites in
leaves and roots of P. pinnata under salt stress
Figure 5 shows the interaction between hormones and metabolites. In
leaves, hormones including ABA, IBA, MeJA, zeatin and JA showed a
positive correlation with MytA in leaves of 300 mM NaCl treated plants
at 1DAS (Figure 5A). Hormones JA, MeJA and zeatin showed significant
interaction with metabolites such as amino acids, carbohydrates, and
organic acids in leaves of 300 mM NaCl treated plants at 4DAS (Figure
5B). In addition, JA and MeJA also showed strong association with
carbohydrates and amino acids in leaves of 300 mM NaCl treated plants at
4DAS. At 8DAS, JA and ABA showed significant correlation with several
metabolites MytA, Suc, Gal, Man and FA, while ABA and JA were showed
significant interaction with metabolites pTag, organic acid group (NA,
Pip, MA, 5Hpip, CAA), amino acids group (Hse, Asp, Asn), StA, and other
metabolites (G3p, NAG, Grl-g) (Figure 5C). In leaves of 500 mM NaCl
treated plants, we could observe more interaction between hormones and
metabolites including MeJA:IBA:zeatin:JA:IAA::carbohydrates,
JA:MeJA:IAA:zeatin::organic acids, ABA:IBA:IAA:zeatin::amino acids,
JA:IBA:IAA:SA:zeatin::polyols and fatty acids at 1DAS (Figure 5D).
Increased interactions were also observed between hormones and
metabolites namely JA:MeJA:zeatin::carbohydrates and
cell-wallcarbohydrates, JA:MeJA:IBA:zeatin::organic acids,
MeJA:IBA:IAA::amino acids, JA:MeJA:zeatin::polyols at 4DAS (Figure 5E).
We recorded a significant positive correlation between hormones and
metabolites namely MeJA:JA:ABA:SA::carbohydrates and cell-wall
carbohydrates, JA:ABA:MeJA:SA::organic acids, JA:ABA:MeJA:IBA:IAA::amino
acids, JA:ABA::polyols, fatty acids and JA:ABA:MeJA:IBA:zeatin::fatty
acids in leaves of 500 mM NaCl treated plants at 8DAS (Figure 5F).
In roots, significant associations between hormones and metabolites were
including JA:SA:ABA:MeJA:IBA:zeatin::carbohydrates,
JA:MeJA:SA:ABA:IAA:SA::organic acids and MeJA:IBA::fatty acids in 300 mM
NaCl treated plants at 1DAS (Figure 5G). At 4DAS, more positive
interactions were seen between hormones and metabolites namely
JA:ABA:ABA:SA:IBA::cell-wall carbohydrates, MeJA:JA:zeatin::polyols and
MeJA:zeatin::fatty acids (Figure 5H). At 8DAS, a strong interaction was
noticed between hormones and metabolites (JA:MeJA:ABA:zeatin::organic
acids, JA:IBA:zeatin::amino acidand JA:zeatin::polyols) (Figure 5I). As
the salinity progressed from day 1 to day 8, the positive interactions
decreased in roots of 500 mM NaCl treated plants (Figure 5J-L). In roots
of 500 mM NaCl treated plants, significant interaction was between
hormones and metabolites namely ABA:SA:IBA:zeatin::organic acids at
1DAS, JA:SA:IBA::cell-wall carbohydrates, ABA:SA:JA:MeJA::organic acids
at 4DAS, and ABA:SA::cell-wall carbohydrates, ABA:MeJA::organic acid at
8DAS respectively.