Quantification of Na+, K+, Ca2+ and Cl- ions
Ion content in leaves and roots of control and salt treated plants were determined as described in Munns et al., (2010) with some minor modifications. The collected samples were oven dried at 70°C for 3 days and ground finely with motor and pestle. For ion extraction, 1 g of finely ground sample powder was taken in 5 ml of aqua regia (concentrated HCl: HNO3, 3:1 v/v) and boiled at 95°C for 60 min. The resulting solution was analysed by using an atomic absorption spectrophotometer (GBC 932, Braeside, Australia). Chloride ion concentration was determined by titrimetric method according to Mohr’s method ( Korkmaz, 2001).