Visualization of Na+ ion specific fluorescence in leaves and roots of P. pinnata
To investigate the pattern of Na+ ion accumulation in leaves and roots of P. pinnata , a cell permeable Na+ specific fluorescent probe CoroNa-Green AM was used (Supplementary Figure 3A-J and Supplementary Figure 4A-T ). Leaf sections of control plants were showed no strong Na+specific fluorescence signal at 1, 4 and 8DAS. Similarly, leaf sections of 300 mM NaCl treated plants were also showed no strong Na+ specific fluorescence signal at 1 and 4DAS, whereas a Na+ specific green florescence was observed in apoplasmic region at 8DAS. Conversely, we could observe a consistent increase in the Na+ fluorescence signal in apoplasmic regions in leaf sections of 500 mM NaCl treated plants at 1, 4 and 8DAS. Further, roots were followed a different pattern of fluorescence in both control and salt treated plants. We could observe no strong fluorescence signal in the cortical apoplasmic regions of control root sections at 1, 4 and 8DAS. However, we could observe a strong fluorescence signal in the cortical apoplasmic regions dose dependently in both 300 and 500 mM NaCl treated root sections at 1, 4 and 8DAS.