Experimental approaches
Effects of cytotoxicity, anti-proliferation, apoptotic induction,
anti-migration and anti-invasion of icariin were evaluated in TNBC cells
and tumor mouse model. The inhibitory effect of icariin on
SIRT6/NF-κB/epithelial-mesenchymal
transition (EMT) signaling pathway was investigated by western-blot and
transcriptomic analysis. The regulatory effect of icariin on tumor
immunosuppressive microenvironment was evaluated.