Cell culture
The human breast cancer cell line MDA-MB-231
(RRID:CVCL_0062),
MDA-MB-453 (RRID:CVCL_0418) mouse mammary carcinoma cell line 4T1
(RRID:CVCL_0125) and
human
mammary epithelium cell line MCF-10A (RRID:CVCL_0598), which were
bought from the American Type Culture Collection (Rockville, MD, USA),
were cultured in DMEM or RPMI 1640 medium containing 10% fetal bovine
serum (FBS, Hyclone, Logan, UT, USA) and 1% antibiotics in 5%
CO2at
37ÂșC.
All human cell lines used in this study have been authenticated using
STR profiling within the last three years. All experiments were
performed with mycoplasma-free cells.