Cell culture
The human breast cancer cell line MDA-MB-231 (RRID:CVCL_0062), MDA-MB-453 (RRID:CVCL_0418) mouse mammary carcinoma cell line 4T1 (RRID:CVCL_0125) and human mammary epithelium cell line MCF-10A (RRID:CVCL_0598), which were bought from the American Type Culture Collection (Rockville, MD, USA), were cultured in DMEM or RPMI 1640 medium containing 10% fetal bovine serum (FBS, Hyclone, Logan, UT, USA) and 1% antibiotics in 5% CO2at 37ÂșC. All human cell lines used in this study have been authenticated using STR profiling within the last three years. All experiments were performed with mycoplasma-free cells.