Cell viability assay
MTT assays were used to assess the viability of icariin-treated cancer
cell. Cells were seeded in a 96-well plate and treated with different
concentrations of icariin for the indicated time. Then, 20
μL
of MTT solution (5 mg/mL in cell culture medium) was added into each
well, and the cells were incubated for another 4 h at 37ºC. Then, 150
μL of DMSO was added to dissolve the
formazan precipitates, and the color absorbance was measured at 570 nm
using a Spectra MAX M5 microplate spectrophotometer (Molecular Devices,
CA, USA). All experiments were conducted in triplicate.