Experimental approaches
Effects of cytotoxicity, anti-proliferation, apoptotic induction, anti-migration and anti-invasion of icariin were evaluated in TNBC cells and tumor mouse model. The inhibitory effect of icariin on SIRT6/NF-κB/epithelial-mesenchymal transition (EMT) signaling pathway was investigated by western-blot and transcriptomic analysis. The regulatory effect of icariin on tumor immunosuppressive microenvironment was evaluated.