Transcriptomic analysis
MDA-MB-231 cells and MCF-10A cells were treated with icariin (0 μM and 20 μM) respectively. After 24 h treatment, the total RNA of MDA-MB-231 and MCF-10A cells were extracted. RNA-seq libraries were prepared using the Illumina HiSeq according to the manufacturer’s instructions. Through screening comparison, H-cluster analysis was used to analyze the expression of differential genes.