Cell viability assay
MTT assays were used to assess the viability of icariin-treated cancer cell. Cells were seeded in a 96-well plate and treated with different concentrations of icariin for the indicated time. Then, 20 μL of MTT solution (5 mg/mL in cell culture medium) was added into each well, and the cells were incubated for another 4 h at 37ºC. Then, 150 μL of DMSO was added to dissolve the formazan precipitates, and the color absorbance was measured at 570 nm using a Spectra MAX M5 microplate spectrophotometer (Molecular Devices, CA, USA). All experiments were conducted in triplicate.