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Bio-specific immobilization of enzymes on electrospun PHB nanofibers
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  • Sun Ah Jang,
  • Ji Hyun Park,
  • Hye Jin Lim,
  • Joon Young Oh,
  • Ki Hyeon Bae,
  • Kyung Jin Lee,
  • Jae-Kwang Song,
  • Dong-Myung Kim
Sun Ah Jang
Korea Research Institute of Chemical Technology
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Ji Hyun Park
Korea Research Institute of Chemical Technology
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Hye Jin Lim
Chungnam National University
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Joon Young Oh
Korea Research Institute of Chemical Technology
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Ki Hyeon Bae
Korea Research Institute of Chemical Technology
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Kyung Jin Lee
Chungnam National University
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Jae-Kwang Song
Korea Research Institute of Chemical Technology
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Dong-Myung Kim
Chungnam National University
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Abstract

Phasins are proteins found on the surface of natural polyhydroxyalkanoate (PHA) granules. Due to their high affinity for PHA, they can potentially be used as a fusion partner to immobilize other proteins. In this study, we investigated the immobilization of a lipase onto electrospun polyhydroxybutyrate nanofibers. Due to a superior surface area-to-volume ratio, PHB nanofibers retained much larger amounts of enzyme than conventional immobilization supports. More importantly, when used in combination with a phasin tag, the enzyme immobilized on PHB nanofibers exhibited markedly higher activity and reusability. Our approach combines the advantageous features of nanofibrous materials and the regio-specificity of biomolecular interactions for the efficient use of enzymes.

Peer review status:POSTED

24 Apr 2020Submitted to Biotechnology and Bioengineering
24 Apr 2020Assigned to Editor
24 Apr 2020Submission Checks Completed