3.1.Chronic toxicity study
All the treatment group ratswere administered with Urai mathiraidrug solution at a dose of (10, 50 and 100mg/kg b.wt) throughout the 90 days found to be no clinical toxicity signs such as physical observations, behavioral changes and other parameters such as body weight, food intake, water intake, respiration, convulsion, tremor, changes in eye and skin colors, etc were observed in the treated group compared to the control group. The observations were measured and summarized in Table 1.
3.1.1. Effect ofUrai mathirai on relative organ body weight
The average and relative organ weight of Urai mathirai orally treated group of animals (at dose of 10, 50 and 100mg/kg b.wt) and control groups showed statistically non-significant differences (P > 0.05). The results revealed that, the internal organs of rats were not adversely affected throughout the treatment. The effect ofUrai mathirai on principal organ weights relative to body weight were presented in Table 2 .
3.2.2 Effect ofUrai mathirai on biochemical parameters
The results of the various biochemical parameters on the experimentally treated rats with the oral administration of the Urai mathirai at a dose of (10, 50 and 100mg/kg b.wt) and normal groups showed statistically non-significant differences (P > 0.05). The results revealed that no abnormal changes in serum biochemical parameters such as albumin, total protein, globulin, Total bilirubin, urea, sodium, creatinine and uric acid levels etc., when compared to control group. The effect of Urai mathirai on biochemical parameters measured and summarized in Table 3