Silencing activity of PTSelectâ„¢-siRNA correlates with the
expression of GOI
To evaluate the relationship between silencing efficiency and
productivity, we chose high, medium and low productivity EpoFc
PTSelectâ„¢-clones, electroporated them with GFP/siRNA1 mRNA, and analyzed
them by flow cytometry (Figure 6a). The cell populations that exhibited
maximum GFP (FITC) signal suppression were analyzed by calculating the
percentage of cells with low to no fluorescence, located at
100 on the x-axis (Figure 5a, insert). The percentage
of cells with maximum GFP suppression (100 on the
x-axis) correlates with their respective GOI expression levels (low
producers, 3.7 and 5%; medium producers, 69 and 64.2%; high producers,
89 and 81%). Thus, there is a correlation between the PTSelectâ„¢-siRNA
silencing activity and the GOI expression.