Silencing activity of PTSelectâ„¢-siRNA correlates with the expression of GOI
To evaluate the relationship between silencing efficiency and productivity, we chose high, medium and low productivity EpoFc PTSelectâ„¢-clones, electroporated them with GFP/siRNA1 mRNA, and analyzed them by flow cytometry (Figure 6a). The cell populations that exhibited maximum GFP (FITC) signal suppression were analyzed by calculating the percentage of cells with low to no fluorescence, located at 100 on the x-axis (Figure 5a, insert). The percentage of cells with maximum GFP suppression (100 on the x-axis) correlates with their respective GOI expression levels (low producers, 3.7 and 5%; medium producers, 69 and 64.2%; high producers, 89 and 81%). Thus, there is a correlation between the PTSelectâ„¢-siRNA silencing activity and the GOI expression.