The regulatory mechanism of nitrate-mediated iron deficiency response is conserved in Arabidopsis thaliana
In order to determine whether nitrate-regulated iron deficiency response is conserved in different species, we conducted a similar iron deficiency experiment in Arabidopsis . After 7 days of treatment, the new Arabidopsis leaves were severely chlorotic under the HN-Fe treatment (Fig. 8a, b), while only slight chlorosis was observed under LN-Fe treatment, with a higher chlorophyll content than HN-Fe treatment (Fig. 8a, b, c). Additionally, we detected the total Fe content of shoots and found that the total Fe content was higher under LN treatment than that under HN treatment (Fig. 8d), which was consistent with the findings in ‘Pinyiensis ’. The above results suggested that nitrate-mediated iron deficiency response is conserved inArabidopsis , and it may be also conserved in other plant species.