The promoter activity analysis of CaNAC2c in response to
HTS and exogenous applied hormones
As cis -elements such as TGACG and ABRE responsive for JA and ABA
responsiveness, respectively, are present in the promoter ofCaNAC2c (pCaNAC2c ), to assay whether stress induction
ofCaNAC2c is determined by its promoter, we assayed activity ofpCaNAC2c against HTS and exogenous application of ABA, jasmonic
acid methyl ester (MeJA), indoleacetic acid (IAA) and gibberellic acid 3
(GA3) and the possible regions responsible for the responsiveness by 5’
series deletion analysis. We found that the response of CaNAC2cto HTS is regulated by ABA via the ABRE (ABA response element)
containing pCaNAC2c261 . In addition,CaNAC2c was upregulated by exogenous applied MeJA, which was
attributed to the TGACG-box locates -1381 to -632bp in pCaNAC2c .
Furthermore, CaNAC2c was downregulated by exogenous applied GA3
or IAA, and the cis -elements responsible for GA3or IAA responsive
might locate within -1381 to -935bp in pCaNAC2c (Figure S3).
These data imply that CaNAC2c is transcriptional regulated by HTS
or RSI via signaling mediated by JA, ABA, IAA or GA3.