Figure.3 The effect of CaNAC2c transient overexpression on thermotolerance and triggered HR mimic cell death antagonized by ABA in pepper leaves. A. the success of CaNAC2c transient overexpression in pepper leaves displayed by western blotting using antibodies against GFP and GFP signal observed under fluorescent confocal microscope at 48 hpi. B. the CaNAC2c transiently overexpressed pepper leaves exhibited an enhanced Fv/Fm compared to that in the control leaves upon HTS.C. the CaNAC2c transiently overexpressed pepper leaves exhibited an enhanced △F/Fm’ compared to that in the control leaves upon HTS. D. thermotolerance related marker genes including CaHSP24 , CaHSP70 and CaHSFB2A were transcriptionally upregulated by transsent overexpression ofCaNCA2C upon HTS.E. the transient overexpression ofCaNAC2c triggered HR mimic cell death under room temperature or by exogenous application of fluridon under HTS, but not under HTS or exogenous application of ABA. F.H2O2accumulation displayed by DAB staining of different organs in CaNAC2c transient overexpression pepper leaves challenged with RSI, HTS and fluridon under HTS. G. the transcription of CaDEF1 andCaHIR1 was upregulated, but CaNPR1 was downregulated by the transient overexpression of CaNAC2c in pepper leaves under room temperature but not under HTS. In C, D and G, data presented are means ± standard error (SE) of four replicates, different letters above the bars indicated significant differences among means (P < 0.01), as calculated with Fisher’s protected LSD test.