Figure.3 The effect of CaNAC2c transient overexpression
on thermotolerance and triggered HR mimic cell death antagonized by ABA
in pepper leaves. A. the success of CaNAC2c transient
overexpression in pepper leaves displayed by western blotting using
antibodies against GFP and GFP signal observed under fluorescent
confocal microscope at 48 hpi. B. the CaNAC2c transiently
overexpressed pepper leaves exhibited an enhanced Fv/Fm compared to that
in the control leaves upon HTS.C. the CaNAC2c transiently
overexpressed pepper leaves exhibited an enhanced △F/Fm’ compared to
that in the control leaves upon HTS. D. thermotolerance related marker
genes including CaHSP24 , CaHSP70 and CaHSFB2A were
transcriptionally upregulated by transsent overexpression ofCaNCA2C upon HTS.E. the transient overexpression ofCaNAC2c triggered HR mimic cell death under room temperature or
by exogenous application of fluridon under HTS, but not under HTS or
exogenous application of ABA. F.H2O2accumulation displayed by DAB staining of different organs in CaNAC2c
transient overexpression pepper leaves challenged with RSI, HTS and
fluridon under HTS. G. the transcription of CaDEF1 andCaHIR1 was upregulated, but CaNPR1 was downregulated by
the transient overexpression of CaNAC2c in pepper leaves under room
temperature but not under HTS. In C, D and G, data presented are means ±
standard error (SE) of four replicates, different letters above the bars
indicated significant differences among means (P <
0.01), as calculated with Fisher’s protected LSD test.