Cell culture
LNCaP cells expressing AR with a novel mutation T878A in the AR ligand
domain, PC3/AR+ cells, a stable expressing
ARWT cell line originally as a sub-line from the
parental cells PC3, and human embryonic kidney cell line HEK293T were
obtained from the Chinese Academy of Medical Sciences (Tianjin, China).
All the cells were maintained in DMEM medium (Life Technologies, Grand
Island, NY) supplemented with 10% fetal bovine serum (FBS, Life
Technologies), 100 U/ml penicillin, and 100 mg/ml streptomycin.