Cell proliferation assay
Cell proliferation was detected by the cell counting kit-8 (CCK-8) assay
according to the manufacturer’s instructions. U937 cells harboring the
LILRA3 overexpression plasmid or the null vector were plated in 96-well
plates (3.0×103cells/well) for 0, 12, 24, 48 and 72
hours. To each sample, 10 μl CCK-8 solutions (Dojindo, kamimashiki gun
Kumamoto, Japan) were added at the indicated time. After incubation at
37°C in humidified 5% CO2 for 1 hour, the optical
density (OD) of each sample was measured at 450 nm using an
auto-microplate reader (Infinite M200, Tecan, Manedorf, Switzerland).
Three parallel replicates were employed for each sample.