Animal experiment
BALB/c mice (14-16 g) were divided into five groups, four of which were the challenge group, and one was the control group, with 12 mice in each group. The H5 subtype influenza virus was diluted to 106EID50. After anesthesia with isoflurane, the virus was dripped into the nasal cavity. Twelve mice in each group were observed, and we recorded the mortality and body weight for 14 days. On the third and fifth day after the attack, we took three mice from each group randomly and collected the heart, liver, spleen, lung, kidney, brain, and turbinate bone to detect the virus titer in each organ.