Animal experiment
BALB/c mice (14-16 g) were divided into five groups, four of which were
the challenge group, and one was the control group, with 12 mice in each
group. The H5 subtype influenza virus was diluted to
106EID50. After anesthesia with
isoflurane, the virus was dripped into the nasal cavity.
Twelve mice in each group were
observed, and we recorded the mortality and body weight for 14 days. On
the third and fifth day after the attack, we took three mice from each
group randomly and collected the heart, liver, spleen, lung, kidney,
brain, and turbinate bone to detect the virus titer in each organ.