Functional enrichment of PDIA4 in glioma
To understand the mechanism of PDIA4 promoting tumor growth and
illustrate the key signaling regulated by PDIA4, we performed GO
functional enrichment analysis.
Data from both TCGA and CGGA were analyzed by Pearson correlation
analysis and genes with |R|>0.6 were collected for
functional enrichment. As a consequence, 408 terms of biological process
(BP), 110 terms of cellular component (CC), 40 terms of molecular
function (MF) were identified from TCGA database, and 140 terms of BP,
56 terms of CC, 15 terms of MF were identified from CGGA database
respectively (Supplementary Table 1, 2). The top 10 terms of BP mainly
enriched functions of neutrophil mediated immune function (Figure 3A,
B). The MF enrichment indicated functions predominantly involved in
transferase activities, cell adhesion and molecule binding (Figure 3C,
D). Meanwhile, genes from CC terms showed significant association with
focal adhesion, cell-substrate junctions and endoplasmic reticulum lumen
(Figure 3E, F). Besides, we also conducted KEGG pathway analysis with
selected genes. The results revealed strong correlation between PDIA4
related genes and important biological signalings, such as protein
processing in endoplasmic reticulum, human immunodeficiency virus 1
infection and apoptosis (Figure 3G, H).