Functional enrichment of PDIA4 in glioma
To understand the mechanism of PDIA4 promoting tumor growth and illustrate the key signaling regulated by PDIA4, we performed GO functional enrichment analysis.
Data from both TCGA and CGGA were analyzed by Pearson correlation analysis and genes with |R|>0.6 were collected for functional enrichment. As a consequence, 408 terms of biological process (BP), 110 terms of cellular component (CC), 40 terms of molecular function (MF) were identified from TCGA database, and 140 terms of BP, 56 terms of CC, 15 terms of MF were identified from CGGA database respectively (Supplementary Table 1, 2). The top 10 terms of BP mainly enriched functions of neutrophil mediated immune function (Figure 3A, B). The MF enrichment indicated functions predominantly involved in transferase activities, cell adhesion and molecule binding (Figure 3C, D). Meanwhile, genes from CC terms showed significant association with focal adhesion, cell-substrate junctions and endoplasmic reticulum lumen (Figure 3E, F). Besides, we also conducted KEGG pathway analysis with selected genes. The results revealed strong correlation between PDIA4 related genes and important biological signalings, such as protein processing in endoplasmic reticulum, human immunodeficiency virus 1 infection and apoptosis (Figure 3G, H).