HPV status
HPV genotyping was carried out using Real-Time PCR (RT-PCR) technique
with HPV Low Risk (6,11) and High Risk (16,18) Typing Real-TM kit. From
the histological samples of 44 patients, 15 patients were found to have
at least one subtype of HPV. HPV was detected in 15 (34%) of cases
(Table 1).
Four patients were found to have two subtypes present from the
histological specimens. One of the patients had Type 6 and Type 11 HPV,
another patient had Type 6 and Type 16 present while two more patients
had type 16 and Type 18 HPV detected (Table 2). None of the patients had
more than two subtypes present.
The most prevalent type of HPV detected in the samples was Type 11 which
was detected in eight (18%) patients. There were five (11%) patients
that were positive for HPV Type 6. Four patients had HPV Type 16 (9%)
while only two (5%) patients had HPV type 18 detected.
Eleven out of 34 (32%) patients with IP had at least one positive HPV
detected. Two out of seven (29%) patients with tumour recurrence had at
least one type of HPV positivity. Two out of three (67%) patients with
IP with SCC had at least one type of HPV detected.
Association of HPV with tumour recurrence
There were seven patients who had tumour recurrence and were classified
as IP with recurrence (Table 1). From this study, we found that only two
out of the seven (29%) patients with tumour recurrence were positive
for HPV. One of the patients had HPV 16 while another patient had HPV 6
and 11. The recurrence rate of inverted papilloma positive for HPV
infection was 15% compared to IPs with negative HPV infection (18%).
The result was not statistically significant (p>0.05).
(Table 3)