Fresh embryo transfer
In the long agonist protocol, a maximum of two embryos was replaced on Day 3 after retrieval under transabdominal ultrasound guidance. Luteal phase support was given by vaginal or intramuscular progesterone at the discretion of the attending physicians. A pregnancy test was carried out 2 weeks after the transfer. All who had a positive pregnancy test had a transvaginal ultrasound scan 2 weeks after the positive pregnancy test (4 weeks after embryo transfer) to identify the presence of a gestation sac with a foetal heart signifying an ongoing pregnancy. All pregnant women were contacted or traced for the pregnancy outcomes after delivery or miscarriage.