AMH assay:
Anti-Mullerian hormone was analyzed by “Beckman Coulter Access II”
enzymatic-immunoassay. In the assay, calibrators, controls and samples
are incubated in microtitration wells which have been coated with
anti-AMH antibody. After serial enzymatic reactions and washing steps,
the degree of enzymatic turnover of the substrate is determined by dual
wavelength absorbance measurement at 450 nm and between 600 and 630 nm.
The absorbance measured is directly proportional to the concentration of
AMH in the samples. A set of AMH calibrators is used to plot a
calibration curve of absorbance versus AMH concentration. The AMH
concentrations in the samples can then be calculated from this
calibration curve.