Figure 2 Inhibitory effect of biuret for allantoinase activity.
Crude extracts were prepared from shoots of 9-day-old rice seedlings
hydroponically grown without biuret. Extracts were incubated at 30ÂșC
with 10 mmol L-1 allantoin, 50 mmol
L-1 Tricine-NaOH (pH8.0), 2mmol L-1MnSO4, and the desired concentration of biuret for
30min. The amount of allantoic acid produced from allantoin was
colorimetorically determined. Same shape symbols indicate a same crude
extract. Crossbars represent the mean value. The means were not
significantly different among treatments at 5% level (One-way ANOVA
with blocking, n = 4).