Confirmation of transgene in biuret hydrolaseoverexpressing rice plants
DNA was extracted from the second leaves of individual T3 plants of biuret hydrolase overexpressing lines. Second leaves were also excised from the wild-type plants to equalize the effects of leaf clipping. The transgene was detected by PCR using the primers 5´-ATGAAGACACTTTCCAGCGC-3´ and 5´-TGGCAAATGCCTCTCAAGG-3´ and Blend Taq polymerase (Toyobo, Osaka, Japan). Plants confirmed to possess biuret hydrolase were used in the analysis.