3.3 Cytotoxic effect of Phormidin
Cytotoxicity test revealed that Phormidin has a median cell line lethal dose (LD50) of 89.65 (µg/mL) against L929 cell lines. Viability percentage was calculated using the formula,
% viability = (OD of Test/OD of Control) x 100, where control OD was 0.993
% viability at various concentrations of the new FAS inhibitor, Phormidin is depicted in the Table 1.
In-vitro cytotoxicity of Phormidin, and Cerulenin and C75 on cultured A549 cells are calculated on the formula as in the previous case and depicted in Table 1 as well.
In-vitro cytotoxic effect of Phormidin on cultured A549 cells is calculated on the formula shown above and the values are given in Table 1. The LD50 value calculated from the above are also shown in the Table 1.