Annexin V-FITC/propidium iodide flow cytometry
quantification of apoptosis was carried out by flowcytometry. Briefly MCF-7 cells were treated with 48h growth media of bacteria bearing construct and bacteria without construct as well as PBS for 48 h and then double stained with Annexin V-FITC/ 7-aminoactinomycin D (7-AAD). Fluorescence was then detected with a FACS Calibur flow cytometer (BD Biosciences, USA). Annexin V+/7-AAD cells were determined as early and Annexin V+/7-AAD+ cells as late apoptotic cells.