Annexin V-FITC/propidium iodide flow cytometry
quantification of apoptosis was carried out by flowcytometry. Briefly
MCF-7 cells were treated with 48h growth media of bacteria bearing
construct and bacteria without construct as well as PBS for 48 h and
then double stained with Annexin V-FITC/ 7-aminoactinomycin D (7-AAD).
Fluorescence was then detected with a FACS Calibur flow cytometer (BD
Biosciences, USA). Annexin
V+/7-AAD− cells were determined as
early and Annexin V+/7-AAD+ cells as
late apoptotic cells.