Method
E. coli BW25133 was transformed with construction of co-expressing
cardiac hormones and GFP. Bacteria bearing constructs were then IV
administered in mice bearing tumors and then tumor localization, as well
as tumor proliferation, invasion and angiogenesis biomarkers (Ki-67,
VEGFR, CD31and MMP9), changes in cytokine profile, suppression of tumor
growth and survival were analyzed.