2.6. Parasitological procedure
The faecal samples were processed at the Parasitology Laboratory, Department of Veterinary Microbiology and Parasitology, Federal University of Agriculture, Abeokuta, Nigeria. Faecal samples were examined for GIP ova and oocysts using centrifugal floatation techniques. Salt /sugar solution at a specific gravity of 1.3g was used as the floatation solution (www.rcv.ac.uk/review/Parasitology/Flotation fluid/General.htm accessed 26/04/2017). Identification of ova and oocysts was made according to the description given by MAFF (1979) and Soulsby (1982). A positive cattle herd is any herd with the presence of one or more GIPs in the faecal samples.