2.6. Parasitological procedure
The faecal samples were processed at the Parasitology Laboratory,
Department of Veterinary Microbiology and Parasitology, Federal
University of Agriculture, Abeokuta, Nigeria. Faecal samples were
examined for GIP ova and oocysts using centrifugal floatation
techniques. Salt /sugar solution at a specific gravity of 1.3g was used
as the floatation solution
(www.rcv.ac.uk/review/Parasitology/Flotation
fluid/General.htm accessed 26/04/2017). Identification of ova and
oocysts was made according to the description given by MAFF (1979) and
Soulsby (1982). A positive cattle herd is any herd with the presence of
one or more GIPs in the faecal samples.