Figure legends
Figure1. (A) Gating strategy for Flowcytometry analysis of PBMCs from lung cancer patients before, during and after chemotherapy compared to healthy control before and After 72 hours of culture.(a) represents the all events from flowcytometry directed to the lymphocyte population. (b) represents the population of the total T cells CD3+, NK cells and NKT cells, (c) represents the population of CD8 cells and CD4. (d) represents the population of NKCD8 cells and NKCD4. (e) represents GzB expression on all mentioned populations. (B) Representative Flowcytometry analysis of CD3, CD8, CD4, NK, NKCD4 and NKCD8 cells from healthy control(a) , lung cancer patients before(b) , during(c) and after induction of chemotherapy (d)(before culture) (C) Representative Flowcytometry analysis of CD3, CD8, CD4, NK, NKCD4 and NKCD8 cells from healthy control(a) , lung cancer patients before(b) , during(c) and after induction of chemotherapy (d) (after culture).
Figure2. Levels of Intracellular GZB expression on CD3, CD8, CD4, NK, NKCD4 and NKCD8 cells from healthy control(a) , lung cancer patients before(b) , during(c) and after induction of chemotherapy (d) after culture in the presence of Con A (5ng/ml) and IL-2 (50ng/ml) and incubated for 72 hrs, then harvested and stained with the same protocol.
Figure3. (A) Statistical analysis of the fold change in the count of CD3 (a) , CD4 (b) , CD8 (c), NK(d) , NKT (e) , NKCD4 (f) and NKCD8(g) cells from lung cancer patients before, during and after induction of chemotherapy (black bars), compared to healthy control (white bars) . (B) Statistical analysis of the fold change in the expression of GZB on CD3 (a) , CD4 (b), CD8(c) , NK (d) and NKT (e) , NKCD4 (f)and NKCD8 (g) cells from lung cancer patients before, during and after induction of chemotherapy (black bars), compared to healthy control (white bars). Data were analyzed using SPSS software and Mann–Whitney or one-way analysis of variance (a nova) with post-hoc for non-parametric analyses, P-values < 0•05 were considered significant (*and ** p ≤ 0.05, p ≤ 0.01).
Figure4. Statistical analysis of the expression of CXCL8, IL-6, IL-1, CCL2, CXCL10, IL-5, CCL11 and CCL4 in the plasma of lung cancer patients before, during and after induction of chemotherapy (black bars), compared to healthy control (white bars). Data were analyzed using SPSS software and Mann–Whitney or one-way analysis of variance (a nova) with post-hoc for non-parametric analyses, P-values < 0•05 were considered significant (*,**and *** p ≤ 0.05, p ≤ 0.01 and, p ≤ 0.001).