Surface and intracellular staining of PBMCs before and after culture
After PBMCs separation using Ficoll, 200,000 cells were separated immediately after counting for surface staining of anti-CD3 (perCP.Cy5.5), anti-CD4 (APC-A) and anti- CD56 (FITC) and intracellular for GzB by using anti-GzB (PE)[13].