Surface and intracellular staining of PBMCs before and after
culture
After PBMCs separation using Ficoll, 200,000 cells were separated
immediately after counting for surface staining of anti-CD3
(perCP.Cy5.5), anti-CD4 (APC-A) and anti- CD56 (FITC) and intracellular
for GzB by using anti-GzB (PE)[13].