GzB expression on T, NK and NKT cell in lung cancer patients and
healthy control before and after 72 hrs of culture with Con-A and IL-2
GzB expression was investigated in CD3, CD4, CD8, NK, NKT, NKCD4, and
NKCD8 cells also by means of flow cytometry before and after culture
with of IL-2 and Con-A. This also was carried out on 4 different groups:
healthy controls and lung cancer patients before, during and after
induction of chemotherapy.
Before culture, it was clear that CD8 and NK populations were the main
secretary cells of GzB, with the remaining cell populations exhibiting
minimal expression levels of it. Patients after induction of
chemotherapy show a significant increase in the secretion of GzB when
compared with healthy control and patients before and during induction
of chemotherapy and this was generally applied for all cell populations.
After culturing, results demonstrated an increase of GzB expression in
all populations of cells in most groups. Expression of GzB on CD3and CD4
cells showed a notable increase in all groups with a less marked
increase in the group of patients after chemotherapy. As of CD8 cells,
results highlighted a striking increase in GzB expression levels before
and during induction of chemotherapy as an effect of culturing in the
activating media. Meanwhile, it demonstrated a moderate increase in the
CTRL and the patients after the chemotherapy group. As for the
expression of GzB on NK cells, percentages were increased significantly
in CTRL only, while, no changes were detected in lung cancer patients
before, during or after induction of chemotherapy after culture.
Meanwhile, GzB expression on NKT cells was increased significantly in
CTRL, while displaying no changes in lung cancer patients before, during
and after induction of chemotherapy. As of NKCD4 secretion, a
significant increase was observed in CTRL, while a significant decrease
was evident in lung cancer patients after induction of chemotherapy.
Finally, NKCD8 was found to be considerably increased in CTRL, while no
changes were noted in lung cancer patients before, during and after
induction of chemotherapy fig 2.