Chemical and reagents
Lymphocyte separation medium (Ficoll paqué TM) was purchased from Lonza (Basel, Switzerland). RPMI 1640 was purchased from Biochrom (Berlin, Germany) human serum was purchased from Bio Whittaker ( Walkersville, MD), L-glutamine was purchased from Life Technologies (Paisley, Scotland), penicillin, streptomycin, Concanavalin A (Con A) and Brefeldin A was purchased from Sigma (Sigma Chemical Co., Munich, Germany), Interleukin-2 (IL-2) were obtained from R&D Systems (Minneapolis, Minnesota, USA). Phosphate buffer saline ( PBS) was obtained from (Verviers, Belgium). CD3 (perCP.Cy5•5), CD3 (APC) clone (RXB-14/IG), CD4 (Allophycocyanin APC-A), clone ( SK3) CD56 (FITC), granzyme B (GzB) Phycoerythrin (PE) clone(RXB-14/IG9), FACS Perm were purchased from (BD Biosciences (BD), San Jose, CA, USA). Sheath Fluid was purchased from (Luminex Corp, Austin, TX, USA).