Subjects populations
In this study, 15 patients of lung cancer and 5 healthy individuals were included. Blood samples were obtained from volunteers and lung cancer patients under treatment at Oncology Department, Tanta University Hospital. All patients are given informed consent under a protocol approved by the Faculty of Medicine Ethical Committee Review Board, Tanta University. Patients were diagnosed according to world health organization (WHO) criteria based on TNM classification[11] . Classification of subjects was performed after the detection of inclusion criteria and exclusion criteria before, during and after induction of chemotherapy.