PBMCs separation
Blood collection was on K2EDTA tubes for plasma
separation, PBMCs were separated using Ficoll
paquƩTM technique in duplicate using break off
centrifugation, twice washing using cold PBS, counting using
hymocytometer to check the viability using trypan blue and to determine
the number of PBMCs in the sample and the actual number of cultured
cells[12] .