Chemical and reagents
Lymphocyte separation medium (Ficoll paqué TM) was
purchased from Lonza
(Basel,
Switzerland). RPMI 1640 was purchased from Biochrom (Berlin, Germany)
human serum was purchased from Bio Whittaker ( Walkersville, MD),
L-glutamine was purchased from Life Technologies (Paisley, Scotland),
penicillin, streptomycin, Concanavalin A (Con A) and Brefeldin A was
purchased from Sigma (Sigma Chemical Co., Munich, Germany),
Interleukin-2 (IL-2) were obtained from R&D Systems (Minneapolis,
Minnesota, USA).
Phosphate buffer saline ( PBS) was obtained from (Verviers, Belgium).
CD3 (perCP.Cy5•5), CD3 (APC) clone (RXB-14/IG), CD4 (Allophycocyanin
APC-A), clone ( SK3) CD56 (FITC), granzyme B (GzB) Phycoerythrin (PE)
clone(RXB-14/IG9), FACS Perm were purchased from (BD Biosciences (BD),
San Jose, CA, USA). Sheath Fluid was purchased from (Luminex Corp,
Austin, TX, USA).