GzB expression on T, NK and NKT cell in lung cancer patients and healthy control before and after 72 hrs of culture with Con-A and IL-2
GzB expression was investigated in CD3, CD4, CD8, NK, NKT, NKCD4, and NKCD8 cells also by means of flow cytometry before and after culture with of IL-2 and Con-A. This also was carried out on 4 different groups: healthy controls and lung cancer patients before, during and after induction of chemotherapy.
Before culture, it was clear that CD8 and NK populations were the main secretary cells of GzB, with the remaining cell populations exhibiting minimal expression levels of it. Patients after induction of chemotherapy show a significant increase in the secretion of GzB when compared with healthy control and patients before and during induction of chemotherapy and this was generally applied for all cell populations.
After culturing, results demonstrated an increase of GzB expression in all populations of cells in most groups. Expression of GzB on CD3and CD4 cells showed a notable increase in all groups with a less marked increase in the group of patients after chemotherapy. As of CD8 cells, results highlighted a striking increase in GzB expression levels before and during induction of chemotherapy as an effect of culturing in the activating media. Meanwhile, it demonstrated a moderate increase in the CTRL and the patients after the chemotherapy group. As for the expression of GzB on NK cells, percentages were increased significantly in CTRL only, while, no changes were detected in lung cancer patients before, during or after induction of chemotherapy after culture.
Meanwhile, GzB expression on NKT cells was increased significantly in CTRL, while displaying no changes in lung cancer patients before, during and after induction of chemotherapy. As of NKCD4 secretion, a significant increase was observed in CTRL, while a significant decrease was evident in lung cancer patients after induction of chemotherapy. Finally, NKCD8 was found to be considerably increased in CTRL, while no changes were noted in lung cancer patients before, during and after induction of chemotherapy fig 2.